The first site for the future Episcopal church in Twin Falls, located at Third Avenue and Second Street North, was selected in the autumn of 1905 by the Rt. Rev. James A. Funston, Boise, Bishop of the Missionary District of Idaho. The site was chosen as an alternative to the one offered by the Twin Falls Townsite Company fronting the four-block city park due to availability of additional adjoining lots.

Bishop Funston designated the Rev. A. F. Randall, rector at Pocatello, to conduct services for the new mission church and the first service was held November 1, 1905, in the schoolhouse at Third Avenue and Third Street East. The next month, Bishop Funston appointed the first vestry including George Sprague, Herbert Osborn, and Stuart Taylor. The communion rail in the present church is a memorial to Mr. Sprague, who was the first to alert the Bishop to the possibility of a church in Twin Falls and who continued to serve as Jr. and Sr. Warden for 25 years.

Early in 1907 the Bishop traveled to Trinity Church in Philadelphia where he successfully solicit enough money from the congregation to build the Twin Falls church. The new church was competed early in the spring of 1908. A large rectory was later built in 1909 on the adjoining lot donated by Mr. Sprague. The Rev. Alvard Chamberlaine was appointed first resident clergy of the Twin Falls mission in May, 1908. Over the 25-year period following the first service, the Church of the Ascension was served by 14 different clergy, roughly a third of the time with no resident pastor. The tireless support of dedicated lay people is credited for sustaining the mission and conducting most of the services.

Ascension Guild proved to be an important factor in sustaining the church both financially and spiritually during these early years. From guild funds this group of about 24 women paid the rector's salary of at least $50 per month, supplied whatever properties they could afford, supported the missionary quota, worked in the choir, purchased hymnals, bought prayer books, paid for janitorial supplies, and after the altar was built, supplied its furnishings.